Basophil allergen threshold sensitivity, and peanut allergen components in relation to DBPCFC in children with suspected peanut allergy
نویسندگان
چکیده
Methods DBPCFC was performed with increasing concentrations of peanut allergen (1 mg to 5 g of peanut) in 42 children with suspected IgE-mediated peanut-allergy. Blood samples were taken for analyses of CD-sens and quantification of IgE-antibodies to peanut and Ara h 1-3, 8-9. Basophils were stimulated in vitro with peanut allergen in descending doses until the threshold sensitivity was reached. CD-sens was defined on the basis of the allergen dose giving 50% of maximal basophil response, measured as expression of CD63. A positive challenge was defined as objective allergic symptoms.
منابع مشابه
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